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WEE1 Interacts with Proto-oncogene, MYC, in Human Esophageal Adenocarcinoma
Location: 54
Mentor: Dr. Zheng Chen
WEE1 is a nuclear protein kinase that plays a vital role in controlling the G2/M checkpoint and cell size during cell cycle regulation. WEE1 functions as a cell cycle inhibitor through the act of phosphorylating and inactivating CDK1 on tyrosine 15 and threonine 14, blocking the early entry into mitosis. The kinase is responsible for regulating CDK2 activity during DNA replication of the S phase and monitoring cell cycle progression to regulate the cell size to ensure proper division. During the progression of human esophageal adenocarcinoma (EAC), WEE1 has been found to be increasingly regulated with concentration being nuclear. A conversion of RNA to cDNA using a reverse transcription protocol was used, along with a Real Time Quantitative Polymerase Chain Reaction (RT-qPCR) of MYC treated genes. WEE1 has been found to have a positive correlation with proto-oncogene, MYC, during infection of Human esophageal adenocarcinoma.
